ABSTRACT
Infection with Helicobacter pylori is the most common cause of stomach and duodenal ulcers. About more than 80% of people are infected with H. pylori in developing countries. H. pylori uses urease enzyme product "ammonia" in order to neutralize and protect itself from the stomach acidic condition and urease enzyme activity has been shown to be essential to the colonization of H. pylori. Inhibitory activity of 20 traditional medicinal plants were examined and evaluated against Jack bean urease activity by Berthelot reaction to obtains natural sources of urease inhibitors. Each herb was extracted using 80% aqueous methanol, then tested its IC[50] value was determined. Eight of the whole 20 studied plants crude extracts were found the most effective with IC[50] values of less than 100 micro g/mL including Laurus nobilis, Zingiber officinale, Nigella sativa, Angelica archangelica, Acorus calamus, Allium sativum,Curcuma longa, and Citrus aurantium extracts, from which most potent urease inhibitory was observed for Zingiber officinale, Laurus nobilis, and Nigella sativa with IC[50] values of 48.54, 48.69 and 59.10 micro g/mL, respectively
ABSTRACT
Helicobacter pylori [H. pylori] infection leads to different clinical and pathological outcomes in humans, including chronic gastritis, peptic ulcer disease and gastric neoplasia and even gastric cancer and its eradiation dependst upon multi-drug therapy. The most effective therapy is still unknown and prompts people to make great efforts to find better and more modern natural or synthetic anti-H. pylori agents. In this report 21 randomly selected herbal methanolic extracts were evaluated for their effect on inhibition of Jack-bean urease using the indophenol method as described by Weatherburn. The inhibition potency was measured by UV spectroscopy technique at 630 nm which attributes to released ammonium. Among these extracts, five showed potent inhibitory activities with IC[50] ranges of 18-35 microg/ mL. These plants are Matricaria disciforme [IC[50]:35 microg/mL], Nasturtium officinale [IC[50]:18 microg/mL], Punica granatum [IC[50]:30 microg/mL], Camelia sinensis [IC[50]:35 microg/mL], Citrus aurantifolia [IC[50]:28 microg/mL]
ABSTRACT
Date is one of the world's oldest food-producing plants wich has always played an important role in the economy and social life. Various researchers examined chemical composition and nutritional values of edible parts of dates while limited information about chemical composition and nutritional quality of date seed is available. In this study, fatty acid composition and total tocopherol content of 14 Iranian date seed oils were studied. Statistical analysis was performed through SPSS computing package. According to the fatty acid profiles, seven fatty acids were found through nearly 50% oleic acid in seeds. Shekar cultivar by 51.40% had the maximum amount and Lasht cultivar by 33.38% had the minimum amount of oleic acid. Tocopherol content in the samples varied between 33.86 microg vit E/g oil for Shahabi2 to 10.09 microg vit E/g oil for Shekar. Tocopherol content was 1.88 and 0.61 microg respectively in one-gram seed of these two cultivars. Iranian date seed oils classified as oleic-lauric oil, had a high amount of oleic acid and could serve as a profitable source of valuable oils for industrial applications